Real-Time PCR (qPCR)

Quality That Counts

With Thermo Fisher's QuantStudio Real-Time PCR platforms, which are built to provide accurate, repeatable, and sensitive nucleic acid analysis, one can have confidence in your outcomes on every run.

• Utilise cutting-edge optical technology and consistent thermal performance to achieve precise quantification.
• Reduce repeated runs as well as increase efficiency and reliability by producing outstanding outcomes on the very initial run.
• Assure reliable assay outcome via clever calibration features along with sturdy engineering.

Productivity That Liberates-

Optimise your molecular operations to ensure everybody involved can concentrate on science as well as diagnostics, which are the most important things.

Multiplexing Capacity

3–4 targets

5–6 targets

6+ colors, 21 filter combinations

Block Format

Quantstudio 3	Quantstudio 5	Quantstudio 7 Pro Flex
96-well fast/standard block (0.1 mL or 0.2 mL)	96-well fast/standard Optional 384-well block (model dependent)	Interchangeable blocks:– 96-well fast/standard – 384-well – TaqMan™ Array Card (for high throughput)

Throughput	QuantStudio 3: ~40 min rapid runs QuantStudio 5: As fast as ~30 min with fast cycling QuantStudio 7 Flex: High-throughput capability; supports automation and TAC cards
Sample Volumes	Reaction volume: 10–100 μL depending on plate type 96-well fast plates: 10–30 μL 384-well plates: 5–20 μL
Optics System	QuantStudio 3- 4-color QuantStudio 5- 5- or 6-color depending on configuration QuantStudio 7 Flex- 6-color high-resolution optics
Sensitivity	Detects ≤ 1 copy depending on assay Detects 1.5-fold differences in expression
Data Management	Onboard storage + Secure Cloud connectivity Wireless and Ethernet connectivity Data types: amplification plots, melt curves, HRM data, allelic discrimination Compliance tools (21 CFR Part 11 enabled on QS5 and QS7 Flex)
Quality Assurance	System self-diagnostics Automatic background calibration Optical/thermal verification options QC reminders, performance alerts, audit trails (varies by model) Software supports melt curve QC, thresholding QC, and baseline correction
Acoustic Noise Level	< 60 dBA during operation (typical for all models)
Connectivity	USB, Wi-Fi, Ethernet Remote monitoring via Thermo Fisher Cloud LIMS integration options (QS5 & QS7 Flex)
Power Consumption	QuantStudio 3: ~350–400 W QuantStudio 5: ~400–450 W QuantStudio 7 Flex: ~500–650 W (depending on block type)

• Optimise turnaround time by using optimised assay protocols and quick cycling blocks.
• Make laboratory setup easier with cloud-enabled analysis and intuitive touchscreen interfaces.
• Adaptable plate layouts and customisable run options can boost lab productivity.

Savings That Empower-

Maximise the finest use of lab amenities with strong design and automation-ready features that lower expenses and boost productivity.

• Lower operating expenses and reagent utilisation with effective thermal cycling.
• Reduce hands-on time with automated data reporting, remote surveillance, and incorporated application.
• Increase system longevity with sturdy hardware designed for high-performance, constant operation.